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The Science Behind EBOO – Mechanisms, Biochemistry & Effects

The Science Behind EBOO – Mechanisms, Biochemistry & Effects

November 27, 2025; By Benjamin Kosubevsky

Oxygen is essential for life, and how well our cells use it often determines our energy, resilience, and overall health. In integrative medicine, therapies that support circulation, detoxification, and oxygen delivery have gained attention for their role in optimizing cellular function. One of the most advanced approaches in this category is EBOO, short for Extracorporeal Blood Oxygenation and Ozonation.

At The Longevity Center FL, EBOO (also known as EB02) is offered through a refined process called EBOOST, which goes beyond traditional ozone therapies by combining blood filtration, oxygenation, ozonation, and ultraviolet (UV/UBI) light therapy. While the experience feels simple to the client, the science happening inside the system is layered and fascinating.

Oxygenation and Mitochondrial Support

Our cells generate energy in tiny structures called mitochondria, which rely heavily on oxygen to fuel ATP production. When oxygen delivery is compromised, whether from poor circulation, inflammation, or environmental stress, mitochondrial function can suffer.

EBOO, it EB02, introduces a concentrated infusion of oxygen directly into circulating blood. This can enhance how much oxygen is available to tissues and cells, potentially improving mitochondrial efficiency and overall energy metabolism. Better oxygenation may translate to sharper mental clarity, steadier energy, and more efficient cellular repair processes.

The Role of Ozone

Ozone (O₃) is a molecule made up of three oxygen atoms, known for its strong oxidative properties. In medical applications, ozone is introduced at carefully controlled doses. Instead of overwhelming the body, this mild oxidative exposure may stimulate adaptive responses.

Research suggests ozone can:

  • Promote antioxidant defenses – Exposure to ozone may encourage the body to upregulate antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. These systems help protect cells from free radical damage.
  • Support immune modulation – Ozone may signal immune cells to balance their activity, which could help calm overactive immune responses while supporting defense against unwanted microbes.
  • Improve circulation – Ozone has been shown to make red blood cells more flexible, which may improve microcirculation and tissue oxygen delivery.

In the context of EBOOST, ozone works in synergy with oxygenation and other steps of the therapy, providing both immediate and signaling effects on the body.

Blood Filtration and Detoxification Support

One of the unique aspects of EBOOST compared to standard ozone IVs is its built-in filtration process. As blood passes through the extracorporeal circuit, certain metabolic byproducts, inflammatory mediators, and other circulating debris can be filtered out.

This filtration may support detoxification by reducing the burden on the liver, kidneys, and lymphatic system. For individuals dealing with high levels of environmental toxins or chronic inflammatory load, this step can be especially important.

The Addition of UV/UBI Light Therapy

After filtration, the blood passes through ultraviolet (UV) light, a step known as Ultraviolet Blood Irradiation (UBI). This technique has been studied for nearly a century and is thought to offer several potential benefits:

  • Pathogen reduction – UV light may help neutralize certain microorganisms, supporting immune resilience.
  • Inflammation modulation – By altering cellular signaling, UBI may calm overactive inflammatory pathways.
  • Antioxidant activation – UV exposure may stimulate the body’s natural antioxidant systems, working hand in hand with ozone to promote redox balance.

The UV/UBI component makes EBOOST distinct from many other EBOO and EB02 systems and may enhance its capacity to support immune and detox pathways simultaneously.

Immune System Signaling

One of the most intriguing effects of EBOO is how it interacts with the immune system. The controlled oxidative stress from ozone and UV exposure may act as a form of “hormesis” — a mild stress that encourages the body to adapt and strengthen its defenses.

This type of signaling may help the immune system become more efficient, responding better to challenges without overreacting. For individuals with chronic immune dysregulation or frequent exposures to environmental stressors, this balancing effect can be an important reason to consider EBOOST.

Circulation and Endothelial Function

Healthy circulation depends on the flexibility of blood vessels and the ability of red blood cells to move smoothly through capillaries. Ozone has been shown to improve red blood cell deformability, while oxygen infusion increases the oxygen content of the blood itself.

Together, these effects may support better delivery of oxygen and nutrients to tissues, potentially improving recovery, vitality, and overall resilience.

Putting the Science Together

EB02 is more than just one therapy — it is a layered process where each step builds on the next:

  • Filtration helps remove circulating waste.
  • UV/UBI light may neutralize pathogens, reduce inflammation, and activate antioxidants.
  • Oxygen infusion increases available oxygen for cellular energy.
  • Ozone exposure stimulates adaptive responses that support immunity and antioxidant balance.

The result is a therapy that interacts with multiple biological systems simultaneously, offering broad potential applications in integrative medicine.

Final Thoughts

It is clear that ozone IV therapy brings together several powerful mechanisms into one streamlined process. By combining oxygenation, ozonation, filtration, and UV/UBI light, EBOO may help support mitochondrial function, immune balance, circulation, detoxification, and antioxidant defenses.

For those seeking advanced approaches to wellness and longevity, EBOOST offers a cutting-edge option that goes beyond standard ozone IV therapy.

To learn more about EBOOST and whether it may fit into your personalized health plan, visit www.thelcfl.com and schedule a consultation with The Longevity Center FL.

Benjamin Kosubevsky

Meet Dr. Benjamin Kosubevsky

Dr. Benjamin Kosubevsky is a board-certified expert in regenerative and integrative medicine. He’s developed his own Ozone Dialysis (EBOO) protocols for more effective, lasting results. With training from leading organizations in ozone, stem cell, and chelation therapies, he offers advanced treatments like PRP, BMAC, and OMT. Dr. Kosubevsky blends traditional and holistic care to create personalized plans that support natural healing. He also co-hosts the Peak Performance Podcast, where he shares insights on health, recovery, and optimal living.